• Ph.D. (Biotechnology, Life Science), Barkatullah University, Bhopal in collaboration with ICAR- High Security Animal Diseases Laboratory (ICAR- HSADL) Bhopal (2013).

• M.V.Sc. (Veterinary Biotechnology), JNKVV Jabalpur (2006).

• B.V.Sc. & A.H. Collage of Veterinary Sciences & Animal Husbandry, JNKVV Jabalpur (2004).

• Qualified ASRB-NET in November 2010.

• Life Member of Society for Bio-safety.
• Life Member of Association of Animal Scientist (AAS).
• Life Member of Indian Association of Veterinary Microbiolog ists, Immunologists, and Specialists in Infectious Diseases (IAVMI).
• Life Member of Veterinary Public Health Specialists.

• Virology, Molecular Biology

1. Nema RK, Singh AK, Joshi A, Shankar P, Gupta S, Yadav AK, Nema S, Mathew BJ, Shrivas A, Patankar C, Raghuwanshi A, Pandey R, Tripathi R, Ansari K, Singh K, Yadav J, Biswas D, Singh S. Testing of four-sample pools offers resource optimization without compromising diagnostic performance of real time reverse transcriptase-PCR assay for COVID-19. PLoS One. 2021; 16(5): e0251891. PMID: 34003869 [IF 3.24].

2. Nema RK, Singh AK, Joshi A, Shankar P, Nema S, Raghuwanshi A, Patankar C, Mathew BJ, Shrivas A, Pandey R, Tripathi R, Biswas D, Singh S (2020). Evaluation of pooled sample analysis strategy in expediting case detection in areas with emerging outbreaks of COVID-19: A pilot study. PLoS One. 15(9):e0239492.

3. Mathew BJ, Vyas AK, Khare P, Gupta S, Nema RK, Nema S, Gupta S, Chaurasiya SK, Biswas D, Singh AK. Laboratory Diagnosis of COVID-19: Current Status and Challenges. Iran J Microbiol. 2021; 13(1): 1-7. PMID: 33889356.

4. Agarwal A, Gupta S, Yadav AK, Nema RK, Ansari K, Biswas D. Molecular and phylogenetic analysis of Chikungunya virus in Central India during 2016 and 2017 outbreaks reveal high similarity with recent New Delhi and Bangladesh strains. Infect Genet Evol. 2019; 75.

5. Mishra N, Pitale SS, Rajukumar K, Prakash A, Behera SP, Nema RK, Dubey SC. Genetic variety of bovine viral diarrhea virus 1 strains isolated from sheep and goats in India. Acta Virol. 2012;56(3):209-15..

6. Mishra N, Rajukumar K, Kalaiyarasu S, Behera SP, Nema RK, Dubey SC Small interfering RNAs targeting viral structural envelope protein genes and the 5ʹ-UTR inhibit replication of bovine viral diarrhea virus in MDBK cells. Acta Virol. 2011;55(3):279-82..

7. Behera SP, Mishra N, Vilcek S, Rajukumar K, Nema RK, Prakash A, Kalaiyarasu S, Dubey SC. Genetic and antigenic characterization of bovine viral diarrhoea virus type 2 isolated from cattle in India. Comp Immunol Microbiol Infect Dis. 2011 Mar;34(2):189-96.

8. Sarma DK, Mishra N, Vilcek S, Rajukumar K, Behera SP, Nema RK, Dubey P, Dubey SC. Phylogenetic analysis of recent classical swine fever virus (CSFV) isolates from Assam, India. Comp Immunol Microbiol Infect Dis. 2011 Jan;34(1):11-5.

9. Mathapati BS, Mishra N, Rajukumar K, Nema RK, Behera SP, Dubey SC. Entry of bovine viral diarrhea virus into ovine cells occurs through clathrin-dependent endocytosis and low pH-dependent fusion. In Vitro Cell Dev Biol Anim. 2010 May; 46(5):403-7. .

10. Mishra N, Mathapati BS, Rajukumar K, Nema RK, Behera SP, Dubey SC. Molecular characterization of RNA and protein synthesis during a one-step growth curve of bovine viral diarrhoea virus in ovine (SFT-R) cells. Res Vet Sci. 2010 Aug; 89(1):130-2.

11. Mishra N, Rajukumar K, Pitale SS, Prakash A, Nema RK, Behera SP, Dubey SC. Evidence of a humoral immune response against the prokaryotic expressed N-terminal autoprotease (N(pro)) protein of bovine viral diarrhoea virus. J Biosci. 2010 Mar; 35(1):79-86..

12. Mishra N, Rajukumar K, Tiwari A, Nema RK, Behera SP, Satav JS, Dubey SC. Prevalence of Bovine viral diarrhoea virus (BVDV) antibodies among sheep and goats in India. Trop Anim Health Prod. 2009 Oct; 41(7):1231-9. .

13. Mishra N, Rajukumar K, Vilcek S, Kalaiyarasu S, Behera SP, Dubey P, Nema RK, Gavade VB, Dubey SC, Kulkarni DD. Identification and molecular characterization of border disease virus (BDV) from sheep in India. Comp Immunol Microbiol Infect Dis. 2016;44:1-7.

14. Dubey P, Mishra N, Rajukumar K, Behera SP, Kalaiyarasu S, Nema RK, Prakash A. Development of a RT-PCR ELISA for simultaneous detection of BVDV-1, BVDV-2 and BDV in ruminants and its evaluation on clinical samples. J Virol Methods. 2015;213:50-6.

15. Behera SP, Mishra N, Nema RK, Pandey PD, Kalaiyarasu S, Rajukumar K, Prakash A. Expression of Bovine Viral Diarrhea Virus Envelope Glycoprotein E2 in Yeast Pichia pastoris and its Application to an ELISA for Detection of BVDV Neutralizing Antibodies in Cattle. J Immunoassay Immunochem. 2015;36(6):639-54.

16. Kalaiyarasu S, Mishra N, Rajukumar K, Nema RK, Behera SP. Development and evaluation of a truncated recombinant NS3 antigen-based indirect ELISA for detection of pestivirus antibodies in sheep and goats. J Immunoassay Immunochem. 2015;36(3):312-23.